Journal: Nanoscale Advances
Article Title: Rapid cellular uptake of citrate-coated iron oxide nanoparticles unaffected by cell-surface glycosaminoglycans †
doi: 10.1039/d4na00277f
Figure Lengend Snippet: SynC uptake and cell-surface interaction with xylosyltransferase-deficient CHO cells. (A) Scheme of wild type CHO-K1 cells and xylosyltransferase-deficient psgA-745 cells. (B) Prussian blue iron staining of wild type and mutant CHO cells grown adherent upon incubation with SynC for 3 h ( n = 6). On average 36 cells were analyzed per condition for each experiment. CHO-K1 had a mean Prussian blue stained area per cell of 31.05% ± 9.58%. PsgA-745 cells had a stained area of 47.60% ± 16.90% per cell (two-tailed unpaired Student's t -test; p = 0.06). (C) Magnetic particle spectroscopy of SynC in the presence of CHO-K1 or psgA-745 cells grown in suspension. Measurements were performed at 12 mT and B off = 0.05 mT at 37 °C for 667 s. N = 4 mean with SD. Measurements normalized to the A 5 / A 3 baseline of SynC in PBS. Wild type CHO-K1 cells caused an A 5 / A 3 increase of Δ12.50% ± 1.06% after 11 s of cell contact. PsgA-745 cells caused an A 5 / A 3 increase of Δ11.92% ± 0.98% within 11 s (two-tailed unpaired Student's t -test; p 11 s = 0.43). (D) Prussian blue iron staining of CHO cells after growth in suspension. Incubation with c Fe = 0.5 mM SynC for 10 min or 60 min. We quantified on average 38 cells per condition for each experiment. No significant differences were determined in SynC uptake by CHO-K1 and psgA-745 cells after growth in suspension (two-tailed unpaired Student's t -test; p 10 min = 0.76; p 60 min = 0.93) ( n = 4). CHO-K1 cells had a mean Prussian blue stained area per cell of 20.45% ± 8.84% after 10 min and 39.62% ± 14.18% after 60 min. Compared to psgA-745 cells 18.30% ± 10.34% (10 min) and 38.55% ± 17.21% (60 min).
Article Snippet: Additionally, we used two Chinese hamster epithelial-like ovary cell lines (CHO): wild type CHO-K1 cells (CCL-61, ATCC) and psgA-745 cells, a xylosyltransferase I/II deficient mutant (CRL2242, ATCC).
Techniques: Staining, Mutagenesis, Incubation, Two Tailed Test, Spectroscopy, Suspension
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